Spots can be excised from fluorescently stained gels using a KBiosystems KCore. The resulting spots are digested robotically with trypsin using a Genomic Solutions ProGest. The digests are cleaned by Ziptip using a Packard Multiprobe IIEX. Peptides and the matrix are spotted automatically to 4700 target plates using the Applied Biosystems Symbiot XVI. A schematic depiction of this process provides a __complete overview__ of this method.

**Example of Process Workflow for Pyruvate Kinase:**

A Peptide Mass Fingerprint from a *Pseudomonas syringae* protein.

The five peaks with red arrows have a MSMS image displayed below in order from largest m/z to smallest. | |

MSMS of m/z 2925 from PMF: | |

MSMS of m/z 2762 from PMF: | |

MSMS of m/z 1827 from PMF: | |

MSMS of m/z 1599 from PMF: | |

MSMS of m/z 1258 from PMF: |

Once the data is submitted for a search, a __MASCOT search result__ is obtained.