Proteomics: Technology Development

We have always been interested in the development of next generation technologies for studying living systems. Beyond the novel bioinformatics tools we work on and the (isobaric tagging) chemistries (for shotgun proteomics) we help report, we are also active in the application of state of the art methods to better understand the molecules that are found in living systems.

Mass spectrometry is an analytical tool for measuring the mass to charge ratio of molecules and we are fortunate to have been one of the first laboratories to employ tandem time of flight mass spectrometry (aka MALDI TOF-TOF). Our laboratory is equipped with a suite of state of the art mass spectrometers to collect information about protein mixtures. Moreover, we are fortunate to have published important papers demonstrating the use of 4plex and 8plex isobaric tagging methods for the quantitation of proteins.

While the application of existing technology is critical to our work, we are also interested in the development of next generation tools. One way in which we do this is to work in the field of nanobiotechnology. By employing the methods and tools of the semiconductor industry, we have developed micro and nanoscale devices for the separation of proteins based on photolithography. These tools have shown the ability to separate mixtures of proteins based on charge, size, affinity, and hydrophobicity and include the ability to inject separated peptides and proteins directly in the electrospray ionization source of a mass spectrometer.

Mass Spectrometers

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