Present - 2008

Complete list of publications:

Publications while at University of Delaware:
2020, 2019, 2018, 2017, 2016, 2015, 2014, 2013, 2012,
2011, 2010, 2009, 2008


Hilliard, WC, Lee, KH. 2020. Systematic identification of safe harbor regions in the CHO genome through a comprehensive epigenome analysis. Biotechnol. and Bioeng. 1– 17.

Ruano-Salguero JS, Lee KH. 2020. Adsorptive-Mediated Endocytosis of Sulfo-Cy5-Labeled IgG Causes Aberrant IgG Processing by Brain Endothelial-Like Cells. Mol Pharm.

Hilliard, WC, MacDonald, ML, Lee, KH. 2020. Cover Image. Biotechnol. and Bioeng., 117: iii-iii.

Hamaker, NK. Lee, KH. 2020. A Site‐Specific Integration Reporter System That Enables Rapid Evaluation of CRISPR/Cas9‐Mediated Genome Editing Strategies in CHO Cells. Biotechnol. J. 15, 2000057.

Hilliard, WC, MacDonald, ML, Lee, KH. 2020. Chromosome‐scale scaffolds for the Chinese hamster reference genome assembly to facilitate the study of the CHO epigenome. Biotechnol. and Bioeng. 117: 2331– 2339.

Ruano-Salguero JS, Lee KH. 2020. Antibody transcytosis across brain endothelial-like cells occurs nonspecifically and independent of FcRn. Sci. Rep. 10:3685.


Dahodwala H, Lee KH. 2019. The fickle CHO: a review of the causes, implications, and potential alleviation of the CHO cell line instability problem. Curr. Opin. Biotechnol. 60:128–137.


Ruano-Salguero JS, Lee KH. 2018. Efflux Pump Substrates Shuttled to Cytosolic or Vesicular Compartments Exhibit Different Permeability in a Quantitative Human Blood-Brain Barrier Model. Mol Pharm. 15(11):5081-5088. doi: 10.1021/acs.molpharmaceut.8b00662.

Baik JY, Lee KH. 2018. Growth Rate Changes in CHO Host Cells Are Associated with Karyotypic Heterogeneity. Biotechnol. J. 13:13–16.

Hamaker NK, Lee KH. 2018. Site-specific integration ushers in a new era of precise CHO cell line engineering. Curr. Opin. Chem. Eng. 22:152–160.

Kremkow BG, Lee KH. 2018. Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool. Metab. Eng. 47:134–142.

MacDonald ML, Hamaker N, Lee KH. 2018. Bioinformatic analysis of Chinese hamster ovary host cell protein lipases. AIChE J. 64:4247–4254.

Rupp O, MacDonald ML, Li S, Dhiman H, Polson S, Griep S, Heffner K, Hernandez I, Brinkrolf K, Jadhav V, Samoudi M, Hao H, Kingham B, Goesmann A, Betenbaugh MJ, Lewis NE, Borth N, Lee KH. 2018. A reference genome of the Chinese hamster based on a hybrid assembly strategy. Biotechnol. Bioeng. 115:2087–2100.

Sawicki LA, Choe LH, Wiley KL, Lee KH, Kloxin AM. 2018. Isolation and Identification of Proteins Secreted by Cells Cultured within Synthetic Hydrogel-Based Matrices. ACS Biomater. Sci. Eng. 4:836–845.

Valente KN, Levy NE, Lee KH, Lenhoff AM. 2018. Applications of proteomic methods for CHO host cell protein characterization in biopharmaceutical manufacturing. Curr. Opin. Biotechnol. 53:144–150.


Baik JY, Lee KH. 2017. A framework to quantify karyotype variation associated with CHO cell line instability at a single-cell level. Biotechnol. Bioeng. 114:1045–1053.

Chiu J, Valente KN, Levy NE, Min L, Lenhoff AM, Lee KH. 2017. Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations. Biotechnol. Bioeng. 114:1006–1015.

Lertsuwan K, Choe LH, Marwa IR, Lee K, Sikes RA. 2017. Identification of Fibulin-1 as a Human Bone Marrow Stromal (HS-5) Cell-Derived Factor That Induces Human Prostate Cancer Cell Death. Prostate 77:729–742.

Rehmann MS, Skeens KM, Kharkar PM, Ford EM, Maverakis E, Lee KH, Kloxin AM. 2017. Tuning and Predicting Mesh Size and Protein Release from Step Growth Hydrogels. Biomacromolecules 18:3131–3142.


Baik, JY, Lee KH (2016). Toward improved host cell protein impurity assessment. Biotechnology J. Jun 22. doi: 10.1002/biot.201600223. PMID: 27331371

Harcum SW, Lee KH. 2016. CHO Cells Can Make More Protein. Cell Syst. 3:412–413.

Heintz KA, Bregenzer ME, Mantle JL, Lee KH, West JL, JH Slater. Fabrication of Three-Dimensional Biomimetic Microfluidic Networks in Hydrogels. In press. 10.1002/adhm.201600351

Levy AT, Lee KH, Hanson TE. 2016. Chlorobaculum tepidum modulates amino acid composition in response to energy availability, as revealed by a systematic exploration of the energy landscape of phototrophic sulfur oxidation. Appl. Environ. Microbiol. 82:6431–6439.

Levy N, Valente K, Lee KH, Lenhoff A (2016). “Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification”. Biotechnol Bioeng. 113(6):1260-72.

Mantle JL, Min L, Lee KH. 2016. Minimum Transendothelial Electrical Resistance Thresholds for the Study of Small and Large Molecule Drug Transport in a Human in Vitro Blood-Brain Barrier Model. Mol. Pharm. 13:4191–4198.


Choi YS, Lee KH (2015). Multiple reaction monitoring assay based on conventional liquid chromatography and electrospray ionization for simultaneous monitoring of multiple cerebrospinal fluid biomarker candidates for Alzheimer's disease. Arch. Pharm. Res. doi 10.1007/s12272-015-0663-y. PMID: 26404792

Min L, Choe LH, and KH Lee (2015). Improved protease digestion conditions for membrane protein detection. Electrophoresis. 36:1690-1698.

Kremkow BG, Baik JY, MacDonald ML, and KH Lee (2015). " 2.0: Genome resources and website updates". Biotechnology Journal. 10: 931-938.

Venkataramanan KP, Min L, Jones SW, Hou S, Ralston MR, Lee KH and ET Papoutsakis (2015). "Complex and extensive post-transcriptional regulation revealed by integrative proteomic and transcriptomic analysis of metabolite stress response in Clostridium acetobutylicum". Biotechnology for Biofuels Biology. 8: 81.

Kremkow BG, Lee KH (2015). Sequencing Technologies for Animal Cell Culture Research. Biotechnology Letters. 37: 55-65.

Valente K, Lenhoff A, and KH Lee (2015). Expression of Difficult-to-Remove Host Cell Protein Impurities During Extended Chinese Hamster Ovary Cell Culture and Their Impact on Continuous Bioprocessing. Biotech. Bioeng. In press.


Valente KN, Schaefer AK, Kempton HR, Lenhoff AM, Lee KH (2014). Recovery of Chinese hamster ovary host cell proteins for proteomic analysis. Biotechnol. J. 9: 87-99.

Levy N, Valente K, Choe LH, Lee KH, Lenhoff A (2014). Identification and Characterization of Host Cell Protein Product-Associated Impurities in Monoclonal Antibody Bioprocessing. Biotechnol. Bioeng. 111: 904-912.

Baik JY, Lee KH (2014). miRNA Expression in CHO: Nature Knows Best. Biotechnology J. 9:459-460.

Wuest, DM, Lee KH (2014). Amyloid-β concentration and structure influences the transport and immunomodulatory effects of IVIG. J. Neurochem. 130: 136-144.

Yuk IM, Zhang JD, Ebeling M, Werz S, Gomez N, Berrera M, Meiringer C, Shao Z, Swanberg J, Lee KH, Luo J, and B Szperalski (2014). Effects of Copper on CHO Cells: Insights from Gene Expression Analyses. Biotechnology Progress. 30: 429-442.

Tuerk A, Lee KH (2014). The Evolving Engineer. AIChE Journal 60: 1956-1963.

Shayan G, Adamiak B, Choe LH, Relkin NR, and Lee KH (2014). Longitudinal Effects of Intravenous Immunoglobulin on Alzheimer’s Cerebrospinal Fluid Proteome. Electrophoresis 35: 1821-1827.

Baik JY, Lee KH (2014). Toward Product Attribute Control: Developments From Genome Sequencing. Current Opinion in Biotechnology. 30: 40-44.

Lee KH, Dordick J (2014). Editorial: Nanobiotechnology. Current Opinion in Biotechnology 28: iv-v.


Hou S, Jones S, Choe LH, Papoutsakis ET, Lee KH (2013). Workflow for quantitative proteomic analysis of Clostridia acetobutylicum ATCC 824 using iTRAQ tags. 61:269-76.

Choi YC, Hou S, Choe LH, Lee KH (2013). Development of a targeted proteomics method for assay of Alzheimer's disease cerebrospinal fluid biomarkers, J. Chrom. B. 930: 129-135.

Kremkow B, Lee KH (2013). Next-generation sequencing technologies and their potential impact on CHO cell-based biomanufacturing, Pharm. Bio. 1: 455-465.

Wuest DM, Wing AM, Lee KH (2013). Membrane Configuration Optimization for a Murine in vitro Blood-Brain Barrier Model. J. Neuroscience Methods. 212: 211-221.


Villamil MA, Liang Q, Chen J, Choi YS, Hou S, Lee KH, Zhuang Z (2012). Serine phosphorylation is critical for the activation of USP1 and its interaction with a WD40-repeat protein UAF1. Biochemistry. 51:9112-23.

Wuest D, Lee KH (2012). Optimization of Endothelial Cell Growth in a Murine in vitro Blood-Brain Barrier Model, Biotechnology Journal 7: 409-417.

Hammond S, Kaplarevic M, Borth N, Betenbaugh M, Lee KH (2012). Chinese Hamster Genome Database: An Online Resource for the CHO Community at , Biotechnology and Bioengineering 109: 1353-1356.

Hammond S, Swanberg J, Polson S, Lee KH (2012). Profiling Conserved MicroRNA Expression in Recombinant CHO Cell Lines Using Illumina Sequencing, Biotechnology and Bioengineering 109: 1371-1375.

Shayan G, Adamiak B, Relkin NR, Lee KH (2012). Longitudinal Analysis of Novel Alzheimer's Disease Proteomic Cerebrospinal Fluid Biomarkers During Intravenous Immunoglobulin Therapy. Electrophoresis 33 1975-1979.

Valente K, Choe LH, Lenhoff A, Lee KH (2012). Optimization of Protein Sample Preparation for Two-Dimensional Electrophoresis. Electrophoresis. 33: 1947-1957.

Shayan G, Felix N, Cho Y, Chatzichristidi M, Shuler ML, Ober C, Lee, KH (2012). Synthesis and Characterization of High-Throughput Nanofabricated Poly(4-hydroxy styrene) Membranes for In vitro Models of Barrier Tissue. Tissue Engineering Part C 18: 667-676.

Gupta P, Swanberg J, Lee KH (2012). A single nucleotide polymorphism in ycdC alters tRNA synthetase expression and results in hypersecretion in Escherichia coli, Biotechnology Progress 28: 646-653.

Esposito DV, Forest RV, Chang Y, Gaillard N, McCandless BE, Hou S, Lee KH, Birkmire RW, Chen JG (2012). Photoelectrochemical Reforming of Glucose to Produce H2 or Syngas using a WO3-based Tandem Cell Device, Energy and Environmental Science 5: 9091-9099.

Munro S, Choe LH, Zinder S, Lee KH, Walker L (2012). Proteomic and Physiological Experiments to Test Thermotoga neapolitana Constraint-Based Model Hypotheses of Carbon Source Utilization, Biotechnology Progress 28: 312-318.

Wuest D, Harcum S, Lee KH (2012). Genomics in Mammalian Cell Culture Bioprocessing, Biotechnology Advances. 7(3):409-17.


Liao PY, Choi YS, Dinman J, Lee KH (2011). The many paths to frameshifting: kinetic modelling and analysis of the effects of different elongation steps on programmed –1 ribosomal frameshifting, Nucleic Acids Research 39: 300-312.

Shayan G, Choi Y, Shusta EV, Shuler ML, Lee KH (2011). Murine In vitro Model of the Blood-Brain Barrier for Evaluating Drug Transport, European Journal of Pharmaceutical Sciences 42: 148-55.

Hammond S, Lee KH (2011). RNA Interference of Cofilin in Chinese Hamster Ovary Cells Improves Recombinant Protein Productivity, Biotechnology and Bioengineering 109: 528-535.

Hammond S, Swanberg JC, Kaplarevic M, and Lee KH (2011). Genomic sequencing and analysis of a Chinese Hamster ovary cell line using Illumina sequencing technology, BMC Genomics 12: 67.

Wu CY, Whye D, Glazewski L, Choe L, Kerr D, Lee KH, Mason RW, Wang W (2011). Proteomic assessment of a cell model of Spinal Muscular Atrophy, BMC Neuroscience 12: 25.

Shayan G, Shuler ML, Lee KH (2011). The Effect of Astrocytes on the Induction of Blood-Brain Barrier Properties in Aortic Endothelial Cells, Biotechnology Progress 27: 1137-1145.

Xu X, Nagarajan H, Lewis NE, Pan Shengkai, Liu X, Chen W, Xie M, Wang W, Hammond S, AndersenM, Neff N, Passarelli B, Koh W, Lee KH, Betenbaugh MJ, Quake SR, Famili I, Palsson BO, Wang J (2011). The Genomic Sequence of the Chinese Hamster Ovary (CHO) K1 Cell Line, Nature Biotechnology 29: 735-741.

Aggarwal K, Lee, KH (2011). Over-expression of cloned RhsA sequences perturbs the cellular translational machinery in E. coli, Journal of Bacteriology 193: 4869-4880.

Wuest D, Hou S, Lee KH (2011). Metabolic Engineering. Comprehensive Biotechnology. Elsevier BV. Second Edition, 2011, Vol. 3, 617-628.

Wu CY, Curtis A, Choi Y, Madae M, Xu MJ, Berg A, Joneja U, Mason RW, Lee KH, Wang W (2011). Identification of the phosphorylation sites in the survival motor neuron protein by protein kinase A, Biochmica et Biophysica Acta 1814: 1134-1139.

Rakauskait, Rasa; Liao, Pei-Yu; Rhodin, Michael; Lee, Kelvin; Dinman, Jonathan (2011). A rapid, inexpensive yeast-based dual-fluorescence assay of programmed -1 ribosomal frameshifting for high throughput screening, Nucleic Acids Research 39: e97.


Liao PY, Lee KH (2010). From SNPs to functional polymorphism: the insight into biotechnological applications, Biochemical Engineering Journal 49: 149-158.

Bechler ME, Doody AM, Racoosin E, Lin L, Lee KH, Brown WJ (2010). The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex, Journal of Cell Biology 190: 45–53.

Choi Y, Choe LH, Lee KH (2010). Recent cerebrospinal fluid biomarker studies of Alzheimer's disease, Expert Review of Proteomics 7: 919-926.


Rudrappa T, Choi YS, Levia DF, Legates DR, Lee KH, Bais HP (2009). Phragmites australis root secreted phytotoxin undergoes photodegradation to execute severe phytotoxicity, Plant Signaling and Behavior 4: 1-8.

Liao PY, Choi YS, Lee KH (2009). FSscan: a mechanism-based program to identify+1 ribosomal frameshift hotspots., Nucleic Acids Research 37: 7302-11.


Liao PY, Gupta P, Petrov A, Dinman J, Lee KH (2008). A new kinetic model reveals the synergistic effect of E-, P- and A-sites on +1 ribosomal frameshifting, Nucleic Acids Research 36: 2619-2629.

Griffiths L, Choe LH, Lee KH, Reardon KF, Orton E (2008). Protein Extraction and Two-Dimensional Gel Electrophoresis of Water- and Lipid-Soluble Proteins From Bovine Pericardium, a Low-Cellularity Tissue, Electrophoresis 29: 4508-4515.

D'Ascenzo M, Choe LH, Lee KH (2008). iTRAQPak: an R Based Analysis and Visualization Package for 8-plex Isobaric Protein Expression Data.
Briefings in Functional Genomics and Proteomics 7: 127-135.

Gupta P, Lee KH (2008). Silent Mutations Result in HlyA Hypersecretion by Reducing Intracellular HlyA Protein Aggregates.
Biotechnology and Bioengineering 101: 967-974.

Park SM, Lee KH, Craighead HC (2008). On-chip coupling of electrochemical pumps and an SU-8 tip for electrospray ionization mass spectrometry.
Biomedical Microdevices. 10: 891-897.

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