How to use:
Click on any of the links below, but it is best to start with the wild type maps (both intracellular and extracellular). If a spot was identified in wildtype, OR in any other strain, it was annotated on the wildtype map assuming the spot was present in wildtype. Only spots unique to other strains are annotated in the other maps (thus the remaining maps have very few spots noted). To get the relevant information, hover the mouse over the spot of interest. A unique code will appear(e.g. R20). Click anywhere on the map to open a pdf file which contains the ID code, protein name, NCBI #, and other information(requires Adobe 5.0 or higher). Search the pdf file for the code to identify the protein.
Note: In some cases a single spot may be related to multiple proteins.
'ACC#' refers to an in-house database at Cornell.
'PSPTO' refers to the RefSeq identifier.
'NCBI ACC#' refers to an NCBInr entry.
'Protein name' refers to the associated NCBI name.
Wild Type - Intracellular
Wild Type - Extracellular
+hrpL Mutant - Intracellular
+hrpL Mutant - Intracellular Low Molecular Weight
+hrpL Mutant - Extracellular
hrpL Deletion Mutant - Intracellular
hrpL Deletion Mutant - Intracellular Low Molecular Weight
hrpL Deletion Mutant - Extracellular
Spots were visualized with Sypro Ruby on a Fuji FLA3000 scanner. Plugs were analyzed by MS and MSMS combined searches using data obtained on an AB4700 TOF/TOF. GPS Explorer hits at confidence >95% (version 3 software) were accepted.
Please contact us via e-mail with any inquiries.