BS: Chemical Engineering, Lehigh University
Chinese Hamster Ovary (CHO) cells have become a popular host cell line for the development of therapeutic proteins used in a variety of diseases, including cancer. Glycosylation, or the addition of saccharide residues to key sites on a therapeutic protein, has proven to one of the key mediators for a biotherapeutic's efficacy and safety in vivo. A large amount of research has been conducted towards the aim of glycosylation modeling. However, these models tend to be extremely large, requiring thousands of reactions and advanced computational systems to function.
Ben Kremkow, also from the Lee lab, has built the foundation for a new method of glycosylation modeling that combines basic kinetics with genomic reconstruction techniques. This model is lightweight, but it does not produce quantitative output. To increase the utility of this model as well as understand the mechanisms behind glycosyltransferase regulation in CHO cells, I study the localization of glycosyltransferases with the aim of giving the model quantitative prediction capabilities. Through transcriptomic, proteomic, and computational methods, the interactions between glycosyltransferases in CHO cells can be effectively studied and quantified to gain new insights into therapeutic protein production.