BS: Biochemistry, Sichuan University
MS: Biochemistry, Sichuan University
PhD: Biochemistry, Iowa State University
Membrane proteins play an important role in cell signaling and transportation. However, the analysis of membrane proteins is challenging. The establishment of a set of protease digest conditions suitable for the proteomic and mass spectrometry (MS) based analysis of membrane proteins is not yet optimized. We will use MS based data and bioinformatic spectral assignment programs like Mascot score and sequence coverage as readout to compare each digestion method. The best digestion condition determined for membrane proteins will be used to study whole cell lysates in the future.