BS: Chemical Engineering, Michigan State University
Many current biological therapeutics are proteins produced in Chinese hamster ovary (CHO) cells, which undergo post-translational modifications, the most common of which is glycosylation. Furthering the knowledge regarding the connection between cell metabolism and product quality, including glycosylation mechanisms of proteins produced in CHO cells is important and not fully understood. The CHO-K1 genome was sequenced in January 2011, permitting the design of siRNA tools specifically targeting the CHO cell with greater ease, increasing the number of genes that can be targeted for either knockdown or overexpression studies. The target genes of interest encode enzymes related to the glycosylation pathway and modified target gene expression will reveal relationships and correlations between the target genes, central carbon metabolism, protein production, and glycosylation patterns. By further understanding the effects of sugar synthesis and carbon metabolism genes on protein production and glycosylation patterns, control of glycosylation will be easier to achieve.